Frog in the Well (井底之蛙): Do not Sit in the well to see the world

 

By Yuyao Liu

This is a Chinese fable by Saint Zhuāng Zǐ, that goes like this: Once upon a time, a frog lived in a small well. He had fresh water to drink and swim in, soft mud to play in, plenty of insects to eat, and high walls to leap around on. To him, the well was a grand and complete world. One day, a turtle from the East China Sea passed by and tried to enter the small well but found that it could not enter. He told the frog, “The sea is vast beyond your imagination. Even if you travelled a thousand miles, you wouldn’t grasp its width. Even if you descended a thousand feet, you wouldn’t comprehend its depth.” Hearing these words, the frog suddenly realized how limited his world had been.

The proverb ” frog in a well” is often used to describe people who have narrow vision and do not understand the vastness of the world. If the frog does not sit in the comfortable well but dares to challenge and jump out of the well, it will see how vast the world is. This philosophical story tells us that when we stay in our comfort zone, our thinking and vision will be limited, and we will lose the exploration of the wider world. When we have the courage to step into new areas, we will find that the richness of the scientific world is far beyond our imagination.

Yuyao Liu

M.Sc. Chemistry

Ph.D. student in the Biometals, Coatings and Devices group at IMDEA Materials Institute. Her academic and professional background reflects multidisciplinary expertise in chemistry, material science and engineering, 3D printing and biology. Her work focuses on biomimetic 3D-printed scaffolds for cartilage, bone and osteochondral regeneration.

Image of the month - March 2025

Calcium deposition stained by alizarin red (“frog in the well”) by Yuyao Liu

In BCD group, we evaluated biomaterials for osteochondral applications, such as magnesium, zinc, titanium, PLA, PCL and so on via different characterizations. Among them, Alizarin Red Stanning is a typical method to check the released calcium deposition during bone regeneration. As shown in the “frog in the well” image, after 21 days of cell culture in osteogenic medium, a dense cell monolayer formed as the background, wherein the frog-like calcium deposition was stained in red, indicating the appearance of successful mineralization. However, “frog in the well” is only a limited vision, we also have PCR to check the expression of osteogenic genes, western blot to check the production of proteins and histological analysis to check the matrix formation, eventually revealing the underlying mechanism of bone regeneration.

Don’t forget to follow us @BCD_IMDEA to keep updated of all the relevant information from the BCD group! 

Additive manufacturing of quasi-isotropic fiber-reinforced composite laminates for biomedical applications

Surface modification of Mg-Zn-Ca alloy by plasma Electrolytic Oxidation for Biodegradable Implants

Effect of surface modification on 3D-printed NiTi alloys for cardiovascular applications